Abstract:Objective:To establish a new determination method of glyphosate in water by ion chromatography. Methods:The experiment was carried out on ICS-2500 ion chromatography apparatus including IonPac AS18 Analytical Column and ED50 Detectors. The injection volume was 500 μL.The running time was 12.5 min at 35 mmol /L KOH and 1.00mL/min flow rate.Results: The linearity of the method is from 0 to 2.0 mg /L, and the equation was A=2.095C-0.009,the correlation coefficient was 0.9998,The detection limit was 0.0006 mg/L.The recoveries were from 94.4% to 97.0%,and RSD was from 0.9% to 2.2%。Conclusion: The experiment is simple,quick,sensitive and reliable.