Abstract: Abstract:ObjectiveTo develop a method for detecting 6 benzyl adenine, gibberellin, forchlorfenuron, thidiazuron, 4 chlorobenzoxy acetic acid and 2,4 dichlorophenoxyacetic acid in vegetables and fruits by using ultra performance liquid chromatography mass/mass spectrometry method (UPLC-MS/MS). MethodsThe samples were extracted by 1%(V/V) acetonitrile-acetic acid, purified by matrix solid phase dispersion method after being dehydrated with dehydration reagents, and eluted by acetonitrile and 0.1% formic acid and 5 mmoL/L ammonium formate solution after being filtered by microporous filter membrane. The samples were separated on a ACQUITY HSS T3(1.8 μm,2.1 mm×100 mm) column, and then analyzed in the multiple reactions monitoring(MRM) mode. The six kinds of plant growth regulators were all detected with negative mode. ResultsThe six kinds of plant growth regulators showed a good linearity in the rang of 1-200 μg/L (r≥0.9913). The detection limits were 0.242-0.508 μg/kg,the recovery rates were 69.1%-105.0% and values of RSD were 1.04%-5.06%. ConclusionThe UPLC-MS/MS method was simple, quick and accurate, and suitable for the detection of six plant growth regulators in vegetables and fruits.
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