Detection on the content of heptaene menadione in health food by high-performance liquid chromatography method
LIN Guobin，HUA Yongyou，NI Lei，LIN Honglin
Fujian Center for Disease Control and Prevention (Fujian Provinceal Key Laboratory of Zoonosis Research；Teaching Base of School of Public Health, Fujian Medical University),Fuzhou, Fujian 350012, China
Abstract：Objective To establish a method for detection the content of heptaene menadione (MK-7) of vitamin K2 in health food by high-performance liquid chromatography method. Methods The samples were extracted with isopropanol and analyzed by high-performance liquid chromatography, separated by C18 column, using methanol + isopropanol + 0.1% acetic acid water = 485 + 500 + 15（methanol，isopropanol and 0.1% acetic acid water was 485，500 and 15）as mobile phase, detected by PDA detector and quantified by external standard method. Results The detection limit was 0.02 mg/kg of the method, the linear ranges were 0.50 mg/L - 10.00 mg/L, the correlation coefficient was 0.9997, adding standard recovery rate was 95.0% - 98.3%, and the relative standard deviation (RSD) was 2.4% in the repeatability test. Conclusion This method is simple, accurate and reliable, and suitable for detecting the content of MK-7 in vitamin K2.
2022, Vol. 28 
