Intervention study on compound antioxidants on α-amanita toxin-induced apoptosis of V79 cells
ZHAO Kangtao1, LIN Wei1，Dawuranjiang·Yilihamu2, CHEN Run1,FU Wusheng1*
1.Fujian Center for Disease Control and Prevention (Fujian Institute of Preventive Medicine)，
Fuzhou Fujian 350012,China； 2.Fujian Medical University，Fuzhou,Fujian 350100,China
Abstract：Objective To study the pro-apoptotic effect of α-amanita toxin on V79 cells and to understand the intervention effect of compound antioxidants vitamin C (VC) and vitamin E (VE) on α-amanita toxin in promoting V79 cell apoptosis. Methods V79 cells were cultured in vitro. Except the control group, the other groups were exposed to α-amanita toxin at a final concentration of 5 μg/mL. In the early intervention group, VC and VE were added at a final concentration of 100 μmol/L at the same time. The late intervention group was also added with the same concentration of VC and VE after 24 hours. Cell viability, cell mitochondrial membrane potential, and cellular MDA (malondialdehyde) and SOD (superoxide dismutase) contents were detected and compared. Results Comparing with the control group, the cell viability in each group decreased and the exposure group was the lowest one. In the results of mitochondrial membrane potential, the number of normal cells of the exposed group was the least one, the number of early intervention group was close to the control group, and the number of late intervention group was less. The apoptosis rate of the poisoned group was significantly higher than that of the control group, and that of the early intervention group was lower than that of the poisoned group and the positive control group. The contents of MDA of the two intervention groups were lower than that of the exposure group, and the contents of SOD were higher than that of the exposure group and the control group. Conclusion α-Amanita toxin can induce the apoptosis of V79 cells, and the antioxidant combination of VC and VE can interfere with its apoptosisinducing effect. Comparing with the later intervention, the effect of early intervention is more obvious.
2022, Vol. 28 
